MD Scientific

Bioseparation and UHPLC: bispecific antibodies

Bioseparation and UHPLC: bispecific antibodies

Aggregate and monomer contents of protein constructs are best analyzed using SEC, and mass spectrometry detection can provide further insight into the different variants. In order to combine these technologies, not only the use of non-volatile salts is crucial. Also, the column material must withstand all runs without shedding and sample carryover. In this study, using a bispecific antibody, we demonstrate that the TSKgel UP-SW3000 colums meets these requirements.

Column used: TSKgel UP-SW3000 30cm L x 4.6mm ID (PN 0023448)

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