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Bioseparation and UHPLC: Characterization of an ADC mimic

Antibodies are useful vehicles for carrying small molecule drugs to their therapeutic targets. Finding the appropriate drug to antibody-ratio (DAR) is crucial for the right balance between therapeutic efficacy and toxicity. Today’s application note shows the use of SEC-MS to analyze the DAR of an antibody-drug conjugate (ADC) mimic. HIC-UV is used as orthogonal method confirming the obtained results. Antibodies are useful vehicles for carrying small molecule drugs to their therapeutic targets. Finding the appropriate drug to antibody-ratio (DAR) is crucial for the right balance between therapeutic efficacy and toxicity. Today’s application note shows the use of SEC-MS to analyze the DAR of an antibody-drug conjugate (ADC) mimic. HIC-UV is used as orthogonal method confirming the obtained results.

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Columns used:

TSKgel SuperSW3000, 4 µm, 2.0 mm ID x 30 cm L (PN 0021485)
TSKgel Butyl-NPR, 2,5 µm, 4,6 mm ID x 10 cm L (PN 0042168)

For further information about the columns, please contact

MD Scientific info@md-scientific.dk – +45 7027  8565 – or at www.md-scientific.dk